Determination of selected amino acids in serum of patients with liver disease

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39902087" target="_blank" >RIV/00216275:25310/16:39902087 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.17219/acem/63751" target="_blank" >http://dx.doi.org/10.17219/acem/63751</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17219/acem/63751" target="_blank" >10.17219/acem/63751</a>

Result language

angličtina

Original language name

Determination of selected amino acids in serum of patients with liver disease

Original language description

Background. The determination of amino acids can be a reliable approach for extended diagnosis of liver diseases. This is because liver disease can be a cause of impaired amino acid metabolism. Therefore, a method for the determination of serum amino acids, applicable for clinical purposes, is necessary. Objectives. The aim of this study was to find differences in the levels of selected amino acids between patients with liver disease and a control group. Material and Methods. Samples of peripheral venous blood were obtained from a group of patients with liver disease (n = 131, 59 women at an average age of 60 years and 72 men at an average age of 52 years) and a control group (n = 105, 47 women at an average age of 62 years and 58 men at an average age of 58 years). Before the separation, the amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde. For the separation, reverse phase column was used. The effluent was monitored with a fluorescence detector. Results. There were significant differences in the concentrations of some amino acids between the patients and the control group, but also between women and men. Correlations between some amino acids and markers of liver blood tests and lipid metabolism were observed. Conclusions. A simple, relatively rapid and selective HPLC method with fluorescence detection for the determination of selected amino acids in serum has been developed.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Advances in Clinical and Experimental medicine

ISSN

1899-5276

e-ISSN

—

Volume of the periodical

25

Issue of the periodical within the volume

6

Country of publishing house

PL - POLAND

Number of pages

13

Pages from-to

1227-1239

UT code for WoS article

000392944200013

EID of the result in the Scopus database

2-s2.0-85006728978