Development of an online solid-phase extraction with liquid chromatography method based on polymer monoliths for the determination of dopamine
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39915935" target="_blank" >RIV/00216275:25310/16:39915935 - isvavai.cz</a>
Result on the web
<a href="http://apps.webofknowledge.com/Search.do?product=WOS&SID=E2PbjPruZIf4crFxEcX&search_mode=GeneralSearch&prID=0248833b-ce7c-42f9-83d0-a58fdb3d5672" target="_blank" >http://apps.webofknowledge.com/Search.do?product=WOS&SID=E2PbjPruZIf4crFxEcX&search_mode=GeneralSearch&prID=0248833b-ce7c-42f9-83d0-a58fdb3d5672</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201600818" target="_blank" >10.1002/jssc.201600818</a>
Alternative languages
Result language
angličtina
Original language name
Development of an online solid-phase extraction with liquid chromatography method based on polymer monoliths for the determination of dopamine
Original language description
Porous polymer monoliths have been used to develop an online solid-phase extraction with liquid chromatography method for determination of dopamine in urine as well as for a continuous monitoring of dopamine in flowing system. A polymerization mixture containing 4-vinylphenylboronic acid monomer has been used to prepare a trapping column based on specific ring formation reaction with dopamine cis-diol functionality. Additionally, a monolithic stationary phase with zwitterion functionality has been used to prepare capillary column for the separation of dopamine. Experimental conditions including molarity, pH, and flow rate of the loading buffer together with a valve switching time have been optimized to provide the highest recovery for dopamine. Experimental setup has been used to determine dopamine in a urine. By using both calibration curve and standard addition method, the dopamine level was determined to be 1.19 and 1.28 mg/L, respectively. Further, we have used experimental design to optimize coupling of two extraction monolithic loops to separation capillary column with monolithic phase for a comprehensive monitoring of dopamine. After multivariate analysis, sample loading flow-rate and a flow-rate of flushing buffer were selected as the most significant variables. Optimized experimental setup was applied to continuously monitor dopamine degradation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA14-22426S" target="_blank" >GA14-22426S: Development of an multifunctional monolithic capillary column with integrated sample focusing, separation, and on-column electrochemical detection</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
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Volume of the periodical
39
Issue of the periodical within the volume
21
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
4107-4115
UT code for WoS article
000389046300006
EID of the result in the Scopus database
2-s2.0-84994472154