Superficially bound acetylcholinesterase based on a chitosan matrix for neurotoxic compound assay by a photographic technique
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F18%3A39910751" target="_blank" >RIV/00216275:25310/18:39910751 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1080/00032719.2017.1381846" target="_blank" >http://dx.doi.org/10.1080/00032719.2017.1381846</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/00032719.2017.1381846" target="_blank" >10.1080/00032719.2017.1381846</a>
Alternative languages
Result language
angličtina
Original language name
Superficially bound acetylcholinesterase based on a chitosan matrix for neurotoxic compound assay by a photographic technique
Original language description
Smartphones scored big success in the last decade and they broke into analytical chemistry as an easy detection system with comparable parameters to standard laboratory equipment. This work focuses on attachment of enzyme acetylcholinesterase (AChE) onto previously activated chitosan. Here, AChE splits alternative substrate indoxyl acetate. Conversion of substrate is blocking or slowing down by inhibitors from which galantamine and tacrine were tested here as model inhibitors with detection limits, 1.1 µM and 0.18 µM, respectively. Measurement procedure was carried out on a 3D three-dimensional printed holder and red-green-blue (RGB) channels served for digital photography evaluation. The method was validated to standard Ellman´s spectrophotometric assay. Long-term stability of immobilized enzyme as well as sensitivity to organic solvents were also tested.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Letters
ISSN
0003-2719
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
1622-1632
UT code for WoS article
000429348300013
EID of the result in the Scopus database
2-s2.0-85044085562