Acetylcholinesterase based dipsticks with indoxylacetate as a substrate for assay of organophosphates and carbamates
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F12%3A43874622" target="_blank" >RIV/60162694:G44__/12:43874622 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1080/00032719.2011.644743" target="_blank" >http://dx.doi.org/10.1080/00032719.2011.644743</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/00032719.2011.644743" target="_blank" >10.1080/00032719.2011.644743</a>
Alternative languages
Result language
angličtina
Original language name
Acetylcholinesterase based dipsticks with indoxylacetate as a substrate for assay of organophosphates and carbamates
Original language description
Enzyme acetylcholinesterase (AChE) is an important part of cholinergic neurotransmission. It is targeted by many toxins such as nerve agents, organophosphates, and carbamate pesticides. Several drugs for treatment of Alzheimer's disease and Myasthenia gravis are also AChE inhibitors. The inhibitory effect can be used for assay purposes. The presented experiment is devoted to the construction of a colorimetric dipstick with immobilized AChE and using indoxylacetate as a chromogenic substrate. Standard qualitative cellulose filter papers, high-performance TLC plates, cotton gauze, and parafilm were chosen as matrices for the testing. The constructed dipsticks were created for assays of paraoxon as a model organophosphate pesticide and neostigmine as a model carbamate. The assessed limit of detection was 10(-7) mol/l for both inhibitors. It responds absolutely by detecting 4 pmol of inhibitor when a sample volume of 40 mu L is considered. Long term stability and optimization of immobiliza
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Letters
ISSN
0003-2719
e-ISSN
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Volume of the periodical
45
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
367-374
UT code for WoS article
000301869400006
EID of the result in the Scopus database
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