Electrochemical biosensor based on acetylcholinesterase and indoxylacetate for assay of neurotoxic compounds represented by paraoxon

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F12%3A00182628" target="_blank" >RIV/62156489:43210/12:00182628 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/12:PU97494 RIV/60162694:G44__/12:43874615
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Electrochemical biosensor based on acetylcholinesterase and indoxylacetate for assay of neurotoxic compounds represented by paraoxon
Original language description
Acetylcholinesterase (AChE) is an enzyme terminating neurotransmission in cholinergic nervous system. It is targeted by several drugs and toxins. From a toxicological point of view, pesticides and nerve agents are probably the most toxic compounds inhibiting AChE activity. On the account of the enzyme inhibition, AChE is suitable for an in vitro assay of inhibitors and analytical devices such as biosensors are known to work on principle of recording of AChE residual activity. Here reported experiment isbased on previously performed colorimetric dipsticks. Presently, it is further improved by an electrochemical assay. The dipsticks are prepared by an immobilization of electric eel AChE into gelatin membrane on cellulose matrix and assay of the enzyme activity by sticking zone with AChE and indoxylacetate as a chromogenic substrate. The zone with immobilized AChE was placed onto screen printed electrode and enzyme reaction was assessed electrochemically using square wave voltammetry. In
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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