Colorimetric dipstick for assay of organophosphate pesticides and nerve agents represented by paraoxon, sarin and VX

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F10%3A00002841" target="_blank" >RIV/62157124:16270/10:00002841 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/10:00002304 RIV/00216208:11160/10:00300631 RIV/25656180:_____/10:#0000009

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Colorimetric dipstick for assay of organophosphate pesticides and nerve agents represented by paraoxon, sarin and VX

Original language description

A dipstick for fast assay of nerve agents and organophosphate pesticides was developed. Indicator pH papers were used as detectors. The principle of the assay is based on enzymatic hydrolysis of acetylcholine into acetic acid and choline by acetylcholinesterase. Acidification of the reaction medium due to accumulation of acetic acid was visible. The colour changed from dark red to yellow as the pH indicator recognized pH shift. Presence of an organophosphate pesticide or a nerve agent results in irreversible inhibition of acetylcholinesterase intercepted on the dipstick. The inhibition stops the enzymatic reaction. The inhibition appears as no change of the medium pH. Three compounds were assayed: paraoxon-ethyl as representative organophosphate pesticides and nerve agents sarin and VX. The achieved limit of detection was 5 x 10(-8) M for paraoxon-ethyl and 5 x 10(-9) M for sarin and VX. Dipsticks were found stable for at least one month. Suitability of these dipsticks for routine assa

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/FR-TI1%2F541" target="_blank" >FR-TI1/541: *Construction of simple dipstickfor organophosphates assay</a><br>

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Talanta

ISSN

0039-9140

e-ISSN

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Volume of the periodical

81

Issue of the periodical within the volume

1-2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

4

Pages from-to

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UT code for WoS article

000276052700095

EID of the result in the Scopus database

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