Nerve Agents Assay Using Cholinesterase Based Biosensor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F09%3A00332920" target="_blank" >RIV/61388963:_____/09:00332920 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/09:00002134 RIV/00216208:11160/09:00210053 RIV/44555601:13440/09:00004904

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Nerve Agents Assay Using Cholinesterase Based Biosensor

Original language description

Electrochemical biosensor based on electric eel acetylcholinesterase (AChE) (EC 3.1.1.7) was performed for assay of nerve agents ? tabun, sarin, soman, cyclosarin, and VX. The biosensor used AChE as biorecognition element. The presence of nerve agents was accompanied by a strong inhibition of AChE activity. Enzyme activity is easily measurable by electrochemical oxidation of thiocholine created from acetylthiocholine (ATChCl) by AChE-catalyzed hydrolysis. The tested nerve agents were successfully assayed. The best limits of detection were achieved for sarin (5.8810-10 M) and VX (8.5110-10 M) after one-minute assay. The biosensor was found long term stable at low as well as laboratory temperature.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

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Project

<a href="/en/project/OVUOFVZ200807" target="_blank" >OVUOFVZ200807: Biosensors for determination of nerve agents and yperites using recombinant proteins and nanotechnology</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electroanalysis

ISSN

1040-0397

e-ISSN

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Volume of the periodical

21

Issue of the periodical within the volume

10

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

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UT code for WoS article

000266537700009

EID of the result in the Scopus database

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