An acetylcholinesterase based chronoamperometric biosensor for fast and reliable assay of nerve agents

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F13%3A43874955" target="_blank" >RIV/60162694:G44__/13:43874955 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/13:PU126046

Result on the web

<a href="http://www.mdpi.com/1424-8220/13/9/11498" target="_blank" >http://www.mdpi.com/1424-8220/13/9/11498</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/s130911498" target="_blank" >10.3390/s130911498</a>

Result language

angličtina

Original language name

An acetylcholinesterase based chronoamperometric biosensor for fast and reliable assay of nerve agents

Original language description

The enzyme acetylcholinesterase (AChE) is an important part of cholinergic nervous system, where it stops neurotransmission by hydrolysis of the neurotransmitter acetylcholine. It is sensitive to inhibition by organophosphate and carbamate insecticides,some Alzheimer disease drugs, secondary metabolites such as aflatoxins and nerve agents used in chemical warfare. When immobilized on a sensor (physico-chemical transducer), it can be used for assay of these inhibitors. In the experiments described herein, an AChE- based electrochemical biosensor using screen printed electrode systems was prepared. The biosensor was used for assay of nerve agents such as sarin, soman, tabun and VX. The limits of detection achieved in a measuring protocol lasting ten minutes were 7.41 x 10-12 mol/L for sarin, 6.31 x 10-12 mol /L for soman, 6.17 x 10-11 mol/L for tabun, and 2.19 x 10-11 mol/L for VX, respectively. The assay was reliable, with minor interferences caused by the organic solvents ethanol, met

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

KA - Militarism

OECD FORD branch

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Project

<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors

ISSN

1424-8220

e-ISSN

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Volume of the periodical

13

Issue of the periodical within the volume

9

Country of publishing house

CH - SWITZERLAND

Number of pages

9

Pages from-to

11498-11506

UT code for WoS article

000328625800021

EID of the result in the Scopus database

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