Why is the hydrolytic activity of acetylcholinesterase pH dependent? Kinetic study of acetylcholine and acetylthiocholine hydrolysis catalyzed by acetylcholinesterase from electric eel
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F18%3A39912589" target="_blank" >RIV/00216275:25310/18:39912589 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1515/znc-2017-0134" target="_blank" >http://dx.doi.org/10.1515/znc-2017-0134</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1515/znc-2017-0134" target="_blank" >10.1515/znc-2017-0134</a>
Alternative languages
Result language
angličtina
Original language name
Why is the hydrolytic activity of acetylcholinesterase pH dependent? Kinetic study of acetylcholine and acetylthiocholine hydrolysis catalyzed by acetylcholinesterase from electric eel
Original language description
The dependence of the activity of acetylcholinesterase from electric eel at a pH value range of 4.8-9.8 (phosphate buffer), regarding acetylcholine and acetylthiocholine hydrolysis, was determined at 25 degrees C, ionic strength of 0.11 M, and initial substrate concentration of 4 mM. At a pH range of 4.8-9.8, the dependences A(pH) form a sigmoid increasing curve with the maximum catalytic activity at a pH range 8 9.5. For acetylcholine hydrolysis, the kinetic reason for such an increase in A consists mainly of an increase in the rate constant k(2) (Michaelis-Menten) model with increasing pH of the reaction mixture. For acetylthiocholine hydrolysis, the kinetic explication of the determined dependence A(pH) is more complicated.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Zeitschrift für Naturforschung C = A Journal of Biosciences
ISSN
0939-5075
e-ISSN
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Volume of the periodical
73
Issue of the periodical within the volume
9-10
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
345-351
UT code for WoS article
000444081500003
EID of the result in the Scopus database
2-s2.0-85049510320