Magnetic macroporous bead cellulose functionalised with recombinant hyaluronan lyase for controllable hyaluronan fragmentation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F18%3A39913177" target="_blank" >RIV/00216275:25310/18:39913177 - isvavai.cz</a>
Result on the web
<a href="http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=6&SID=C2pzRncJPo1FecCVZHU&page=1&doc=1" target="_blank" >http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=6&SID=C2pzRncJPo1FecCVZHU&page=1&doc=1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.procbio.2018.06.025" target="_blank" >10.1016/j.procbio.2018.06.025</a>
Alternative languages
Result language
angličtina
Original language name
Magnetic macroporous bead cellulose functionalised with recombinant hyaluronan lyase for controllable hyaluronan fragmentation
Original language description
Hyaluronic acid (HA) is a component of the extracellular matrix with important potential for biotechnological and pharmaceutical applications. However, the immunological effects of HA are strongly molecular weight-dependent; therefore, production of HA for therapeutic purposes requires control over the molecular weight of the product, as well as mild reaction conditions and elimination of bioactive impurities. Here, we produced and characterised biocompatible magnetic macroporous bead cellulose (MBC) functionalised with Streptococcus pneumoniae hyaluronan lyase (SpnHL) that are compatible with these requirements and optimised their reaction and storage conditions. Immobilisation of SpnHL on MBC via reductive amination or MBC with fixed iminodiacetic acid (MBC-IDA) via a His(8)-tag had minimal impact on its catalytic activity. The MBC-IDA-SpnHL carrier showed excellent operational and storage stability, and both carriers enabled reproducible time-controlled fragmentation of highly viscous HMW HA solutions, yielding HA fragments of appropriate molecular weight. Thus, these carriers are suitable for numerous industrial and biotechnological applications.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Process biochemistry
ISSN
1359-5113
e-ISSN
—
Volume of the periodical
72
Issue of the periodical within the volume
72
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
105-111
UT code for WoS article
000442710600013
EID of the result in the Scopus database
2-s2.0-85049564986