Determination of amines using HPLC with fluorescence detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F18%3A39913553" target="_blank" >RIV/00216275:25310/18:39913553 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of amines using HPLC with fluorescence detection

Original language description

A simple and sensitive method for the determination of free aliphatic and aromatic amines using derivatives agents (dansyl chloride, 9-fluorenylmethyl chloroformate and 4-Chloro-7-nitrobenzofurazan) as a labelling reagent by high-performance liq-uid chromatography with fluorescence detection (HPLC-FLD) has been developed. Derivatization conditions including reagent concentration, buffer pH, reaction time and temperature were optimized. A fluorescence derivatization - high-performance liquid chromatography (HPLC) method, which enables the femtomole-level detection of analytes, is a powerful tool for the analysis with high sensitivity and selectivity. In recent decades, improvised explosive devices (IEDs) have become an increasing topic of public concern, with high-profile incidents such as the Bali (2002), London transport (2005), Boston Marathon (2013) and Brussels (2016) bombings garnering mass international attention.[1] Although the majority of media coverage has fo-cused on large-scale incidents, there has also been a rise in explosive incidents in-volving smaller devices such as pipe bombs. This is particularly the case in the Unit-ed States, where pipe bombs are considered to make up the vast majority of IED en-counter. These devices can be easily constructed from everyday materials.[2] The mixture nitromethane with amines is applicable for this. Determination of resi-dues after an explosion is important. This method (HPLC-FLD) could be useful for their determination. The proposed method is sensitive and reproducible for the de-termination of aliphatic and aromatic amines.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/VI20152020004" target="_blank" >VI20152020004: Identification of residues of improvised explosives using physico-chemical analytical methods under real conditions</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the 21st Seminar on New Trends in Research of Energetic Materials

ISBN

978-80-7560-136-0

ISSN

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e-ISSN

neuvedeno

Number of pages

5

Pages from-to

533-537

Publisher name

Univerzita Pardubice

Place of publication

Pardubice

Event location

Pardubice

Event date

Apr 18, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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