Determination of hydroxyproline using ion-exchange liquid chromatography with VIS detector and high performance liquid chromatography with fluorescence detector

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912631" target="_blank" >RIV/62156489:43210/17:43912631 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/17:PU128373
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Determination of hydroxyproline using ion-exchange liquid chromatography with VIS detector and high performance liquid chromatography with fluorescence detector
Original language description
The first aim of the experiment was an optimization of the method for determination of hydroxyproline in a pig skin using high-performance liquid chromatography with fluorescence detector (HPLC-FLD) and ion-exchange liquid chromatography with VIS detector (IEC-VIS). On the basis of the experiments performed, it was found that HPLC-FLD method is three times more sensitive than IEC-VIS method. For IEC analysis of hydroxyproline, the limit of detection (LOD) was 4.10 μg/ml whereas the limit of quantification (LOQ) was 13.50 μg/ml. For HPLC analysis of hydroxyproline, the limit of detection (LOD) was 1 ng/ml whereas the limit of quantification (LOQ) was 3 ng/ml. The second aim was the testing of influence of different volume of 6M HCl on extraction of sample (50 mg) for analysis using HPLC-FLD. Here it was found that the best volume was 250 µl 6M HCl.
Czech name
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Czech description
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Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2017: Proceedings of International PhD Students Conference
ISBN
978-80-7509-529-9
ISSN
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e-ISSN
neuvedeno
Number of pages
6
Pages from-to
905-910
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 8, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000440194500162

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