Determination of hydroxyproline using ion-exchange liquid chromatography with vis detector and high performance liquid chromatography with fluorescence detector

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F17%3APU128373" target="_blank" >RIV/00216305:26620/17:PU128373 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/17:43912631

Result on the web

<a href="https://mendelnet.cz/artkey/mnt-201701-0104_Determination-of-hydroxyproline-using-ion-exchange-liquid-chromatography-with-VIS-detector-and-high-performance.php?back=/magno/mnt/2017/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201701-0104_Determination-of-hydroxyproline-using-ion-exchange-liquid-chromatography-with-VIS-detector-and-high-performance.php?back=/magno/mnt/2017/mn1.php?secid=4</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of hydroxyproline using ion-exchange liquid chromatography with vis detector and high performance liquid chromatography with fluorescence detector

Original language description

The first aim of the experiment was an optimization of the method for determination of hydroxyproline in a pig skin using high-performance liquid chromatography with fluorescence detector (HPLC-FLD) and ion-exchange liquid chromatography with VIS detector (IEC-VIS). On the basis of the experiments performed, it was found that HPLC-FLD method is three times more sensitive than IEC-VIS method. For IEC analysis of hydroxyproline, the limit of detection (LOD) was 4.10 μg/ml whereas the limit of quantification (LOQ) was 13.50 μg/ml. For HPLC analysis of hydroxyproline, the limit of detection (LOD) was 1 ng/ml whereas the limit of quantification (LOQ) was 3 ng/ml. The second aim was the testing of influence of different volume of 6M HCl on extraction of sample (50 mg) for analysis using HPLC-FLD. Here it was found that the best volume was 250 µl 6M HCl.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

MendelNet 2017

ISBN

978-80-7509-529-9

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

905-910

Publisher name

Mendel University in Brno

Place of publication

Neuveden

Event location

Brno

Event date

Nov 8, 2017

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

000440194500162