Liquid Chromatography | Multidimensional
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F19%3A39914819" target="_blank" >RIV/00216275:25310/19:39914819 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/B9780124095472144944?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/B9780124095472144944?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/B978-0-12-409547-2.14494-4" target="_blank" >10.1016/B978-0-12-409547-2.14494-4</a>
Alternative languages
Result language
angličtina
Original language name
Liquid Chromatography | Multidimensional
Original language description
In clinical, pharmaceutical, food, natural and environmental analysis, molecular biology and elsewhere, the samples may contain even millions of compounds, in concentrations possibly spanning over 10 orders of magnitude. The maximum theoretical number of peaks that we can separate in a single run HPLC hardly can exceed 100. Combining two or more different separation columns, the theoretical peak capacity, that is, the maximum number of peaks separated in a fixed time can be significantly increased. A chromatographic column together with the operating conditions affecting the retention and selectivity of separation, such as mobile phase, temperature, pH, gradient, etc., represent the separation medium. In one-dimensional separation systems, a single separation medium is used. A system consisting of several separation media is multidimensional (MD), when the separation mechanisms in each dimension are different With any additional separation dimension, the number of resolved compounds, but also the experimental complexity of the MD system and the time requirements dramatically increase. So far, two-dimensional liquid chromatography (2D LC) systems are used in practice; three-dimensional (3D LC) systems are investigated. In addition to major increase in peak capacity, multidimensional separations may provide group separations of various samples based on molecular structure similarities and differences.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Encyclopedia of Analytical Science
ISBN
978-0-08-101983-2
Number of pages of the result
8
Pages from-to
154-161
Number of pages of the book
5109
Publisher name
Elsevier Science BV
Place of publication
Amsterdam
UT code for WoS chapter
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