Colloidally stable polypeptide-based nanogel: Study of enzyme-mediated nanogelation in inverse miniemulsion

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F19%3A39914952" target="_blank" >RIV/00216275:25310/19:39914952 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/20:00522680
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1002/app.48725" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1002/app.48725</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/app.48725" target="_blank" >10.1002/app.48725</a>

Result language
angličtina
Original language name
Colloidally stable polypeptide-based nanogel: Study of enzyme-mediated nanogelation in inverse miniemulsion
Original language description
The current work presents a pivotal study of the nanogelation of the linear poly(N-5-2-hydroxypropyl-L-glutamine) polymer precursor containing tyramine (TYR) units in an inverse miniemulsion by horseradish peroxidase/H2O2-mediated crosslinking. The effects of various n(H2O2)/n(TYR) ratios on the kinetics of nanogelation in the inverse miniemulsion and on the reaction time are investigated by linear sweep voltammetry, while the formation of dityramine crosslinking is explored by fluorescence spectroscopy. The study is completed using dynamic light scattering measurements, nanoparticle tracking analysis, and cryogenic transmission electron microscopy to acquire comprehensive information about the formed nanoparticulate systems. With the optimal ratio n(H2O2)/n(TYR) = 2, the strategy yields in the high-quality 130 nm poly(amino acid)-based nanogel, which is prepared in 2 h. The nanogel is colloidally stable under different temperature and pH conditions for over 168 h. Moreover, the demonstrated nanogel is noncytotoxic for HeLa cells and human primary fibroblasts and is quickly enzymatically hydrolyzed into small fragments during a biodegradation study in human blood plasma. (c) 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 137, 48725.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21001 - Nano-materials (production and properties)

Project
<a href="/en/project/GA18-03224S" target="_blank" >GA18-03224S: INJECTABLE POLYPEPTIDE BASED HYDROGELS MIMICKING THE DYNAMIC PROCESSES IN EXTRACELLULAR MATRIX</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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