Colloidally stable polypeptide‐based nanogel: study of enzyme‐mediated nanogelation in inverse miniemulsion

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F20%3A00522680" target="_blank" >RIV/61389013:_____/20:00522680 - isvavai.cz</a>
Alternative codes found
RIV/00216275:25310/19:39914952
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/app.48725" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/app.48725</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/app.48725" target="_blank" >10.1002/app.48725</a>

Result language
angličtina
Original language name
Colloidally stable polypeptide‐based nanogel: study of enzyme‐mediated nanogelation in inverse miniemulsion
Original language description
The current work presents a pivotal study of the nanogelation of the linear poly(N5‐2‐hydroxypropyl‐L‐glutamine) polymer precursor containing tyramine (TYR) units in an inverse miniemulsion by horseradish peroxidase/H2O2‐mediated crosslinking. The effects of various nH2O2/nTYR ratios on the kinetics of nanogelation in the inverse miniemulsion and on the reaction time are investigated by linear sweep voltammetry, while the formation of dityramine crosslinking is explored by fluorescence spectroscopy. The study is completed using dynamic light scattering measurements, nanoparticle tracking analysis, and cryogenic transmission electron microscopy to acquire comprehensive information about the formed nanoparticulate systems. With the optimal ratio nH2O2/nTYR = 2, the strategy yields in the high‐quality ~ 130 nm poly(amino acid)‐based nanogel, which is prepared in 2 h. The nanogel is colloidally stable under different temperature and pH conditions for over 168 h. Moreover, the demonstrated nanogel is noncytotoxic for HeLa cells and human primary fibroblasts and is quickly enzymatically hydrolyzed into small fragments during a biodegradation study in human blood plasma.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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