Lipidomic characterization of exosomes isolated from human plasma using various mass spectrometry techniques

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F20%3A39916271" target="_blank" >RIV/00216275:25310/20:39916271 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/19:73599350 RIV/00216208:11110/20:10411527

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S1388198120300263" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1388198120300263</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbalip.2020.158634" target="_blank" >10.1016/j.bbalip.2020.158634</a>

Result language

angličtina

Original language name

Lipidomic characterization of exosomes isolated from human plasma using various mass spectrometry techniques

Original language description

Ultrahigh-performance supercritical fluid chromatography - mass spectrometry (UHPSFC/MS), ultrahigh-performance liquid chromatography - mass spectrometry (UHPLC/MS), and matrix-assisted laser desorption/ionization (MALDI) - MS techniques were used for the lipidomic characterization of exosomes isolated from human plasma. The high-throughput methods UHPSFC/MS and UHPLC/MS using a silica-based column containing sub-2 mu m particles enabled the lipid class separation and the quantitation based on exogenous class internal standards in &lt; 7 minute run time. MALDI provided the complementary information on anionic lipid classes, such as sulfatides. The nontargeted analysis of 12 healthy volunteers was performed, and absolute molar concentration of 244 lipids in exosomes and 191 lipids in plasma belonging to 10 lipid classes were quantified. The statistical evaluation of data included principal component analysis, orthogonal partial least square discriminant analysis, S-plots, p-values, T-values, fold changes, false discovery rate, box plots, and correlation plots, which resulted in the information on lipid changes in exosomes in comparison to plasma. The major changes were detected in the composition of triacylglycerols, diacylglycerols, phosphatidylcholines, and lysophosphatidylcholines, whereby sphingomyelins, phosphatidylinositols, and sulfatides showed rather similar profiles in both biological matrices.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GA18-12204S" target="_blank" >GA18-12204S: Characterization of human lipidome and metabolome for personalized healthcare and biomarker discovery: case study of kidney cancer</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

ISSN

1388-1981

e-ISSN

—

Volume of the periodical

1865

Issue of the periodical within the volume

5

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

158634

UT code for WoS article

000520939600003

EID of the result in the Scopus database

2-s2.0-85078489204