The Effect of Repeated Passaging on the Susceptibility of Human Proximal Tubular HK 2 Cells to Toxic Compounds

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F20%3A39916286" target="_blank" >RIV/00216275:25310/20:39916286 - isvavai.cz</a>
Result on the web
<a href="https://www.biomed.cas.cz/physiolres/pdf/2020/69_731.pdf" target="_blank" >https://www.biomed.cas.cz/physiolres/pdf/2020/69_731.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.33549/physiolres.934491" target="_blank" >10.33549/physiolres.934491</a>

Result language
angličtina
Original language name
The Effect of Repeated Passaging on the Susceptibility of Human Proximal Tubular HK 2 Cells to Toxic Compounds
Original language description
The human proximal tubular HK-2 cell line is an immortalized cell line commonly used for studying proximal tubular toxicity. Even as their use is presently increasing, there unfortunately are no studies focused on functional changes in HK-2 cells associated with passaging. The aim of the present study, therefore, was to evaluate the functional stability of HK-2 cells during 13 weeks of continuous passaging after 6 and 24 h of treatment with model nephrotoxic compounds (i.e., acetaminophen, cisplatin, CdCl2). Short tandem repeat profile, the doubling time, cell diameter, glutathione concentration, and intracellular dehydrogenase activity were measured in HK-2 cells at each tested passage. The results showed that HK-2 cells exhibit stable morphology, cell size, and cell renewal during passaging. Mean doubling time was determined to be 54 h. On the other hand, we observed a significant effect of passaging on the susceptibility of HK-2 cells to toxic compounds. The largest difference in results was found in both cadmium and cisplatin treated cells across passages. We conclude that the outcomes of scientific studies on HK-2 cells can be affected by the number of passages even after medium-term cultivation and passaging for 13 weeks.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10601 - Cell biology

Project
<a href="/en/project/GA19-11867S" target="_blank" >GA19-11867S: Research on toxicity mechanism of S-conjugates of aminophenolic drugs</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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