Intra-laboratory comparison of four analytical platforms for lipidomic quantitation using hydrophilic interaction liquid chromatography or supercritical fluid chromatography coupled to quadrupole - time-of-flight mass spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F21%3A39917842" target="_blank" >RIV/00216275:25310/21:39917842 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/21:73608747 RIV/00098892:_____/21:N0000152
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0039914021002885?token=9D7D8381A1F939821CAB5CDF86975DBFBF9A3C67038B782FE53F8C601D881E0AA52CC572CC334C501E2F111B54B1BDCA&originRegion=eu-west-1&originCreation=20211215104430" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0039914021002885?token=9D7D8381A1F939821CAB5CDF86975DBFBF9A3C67038B782FE53F8C601D881E0AA52CC572CC334C501E2F111B54B1BDCA&originRegion=eu-west-1&originCreation=20211215104430</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2021.122367" target="_blank" >10.1016/j.talanta.2021.122367</a>
Alternative languages
Result language
angličtina
Original language name
Intra-laboratory comparison of four analytical platforms for lipidomic quantitation using hydrophilic interaction liquid chromatography or supercritical fluid chromatography coupled to quadrupole - time-of-flight mass spectrometry
Original language description
The lipidomic research is currently devoting considerable effort to the harmonization that should enable the generation of comparable and accurate quantitative lipidomic data across different laboratories and regardless of the mass spectrometry-based platform used. In the present study, we systematically investigate the effects of the experimental setup on quantitative lipidomics data obtained by two lipid class separation approaches, hydrophilic interaction liquid chromatography (HILIC) and ultrahigh-performance supercritical fluid chromatography (UHPSFC), coupled to two different quadrupole - time of flight (QTOF) mass spectrometers from the same vendor. This approach is applied for measurements of 268 human plasma samples of healthy volunteers and renal cell carcinoma patients resulting in four data sets. We investigate and visualize differences among these data sets by multivariate data analysis methods, such as principal component analysis (PCA), orthogonal partial least square discriminant analysis (OPLS-DA), box plots, and logarithmic correlations of molar concentrations of individual lipid species. The results indicate that even measurements in the same laboratory for the same samples using different analytical platforms may yield slight variations in the molar concentrations determined. The normalization to a reference sample with defined lipid concentrations can further diminish these small differences, resulting in highly homogenous molar concentrations of individual lipid species. This strategy indicates a potential approach towards the reporting of comparable quantitative results independent from the quantitative approach and mass spectrometer used, which is important for a wider acceptance of lipidomics data in various biomarker inter-laboratory studies and ring trials.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA18-12204S" target="_blank" >GA18-12204S: Characterization of human lipidome and metabolome for personalized healthcare and biomarker discovery: case study of kidney cancer</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Talanta
ISSN
0039-9140
e-ISSN
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Volume of the periodical
231
Issue of the periodical within the volume
August
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
122367
UT code for WoS article
000658306800024
EID of the result in the Scopus database
2-s2.0-85104073129