Reversed-Phase Ultrahigh-Performance Liquid Chromatography–Mass Spectrometry Method for High-Throughput Lipidomic Quantitation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F24%3A39921987" target="_blank" >RIV/00216275:25310/24:39921987 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007/978-1-0716-4116-3_12" target="_blank" >https://link.springer.com/protocol/10.1007/978-1-0716-4116-3_12</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-4116-3_12" target="_blank" >10.1007/978-1-0716-4116-3_12</a>
Alternative languages
Result language
angličtina
Original language name
Reversed-Phase Ultrahigh-Performance Liquid Chromatography–Mass Spectrometry Method for High-Throughput Lipidomic Quantitation
Original language description
Reversed-phase ultrahigh-performance liquid chromatography-mass spectrometry (RP-UHPLC/MS) method is optimized for the quantitation of a large number of lipid species in biological samples, primarily in human plasma and serum. The method uses a C18 bridged ethylene hybrid (BEH) column (150 × 2.1 mm; 1.7 μm) for the separation of lipids from 23 subclasses with a total run time of 25 min. Lipid species separation allows the resolution of isobaric and isomeric lipid forms. A triple quadrupole mass spectrometer is used for targeted lipidomic analysis using multiple reaction monitoring (MRM) in the positive ion mode. Data are evaluated by Skyline software, and the concentrations of analytes are determined using internal standards per each individual lipid class.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10400 - Chemical sciences
Result continuities
Project
<a href="/en/project/GA21-20238S" target="_blank" >GA21-20238S: Multidimensional chromatography - mass spectrometry quantitative workflows in detailed characterization of human plasma lipidome</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Clinical Metabolomics : Methods and Protocols
ISBN
978-1-4939-7592-1
Number of pages of the result
9
Pages from-to
185-194
Number of pages of the book
575
Publisher name
Humana Press
Place of publication
Totowa
UT code for WoS chapter
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