Lipidomics: Supercritical fluid chromatography/ion mobility - mass spectrometry as a tool for fast nontargeted analysis of lipids

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899334" target="_blank" >RIV/00216275:25310/14:39899334 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Lipidomics: Supercritical fluid chromatography/ion mobility - mass spectrometry as a tool for fast nontargeted analysis of lipids
Original language description
Individual parameters of SFC analysis have been carefully optimized to achieve a maximum number of separated lipid classes. Developed SFC method enables the fast separation of 16 nonpolar and polar lipid classes within 6 minute analysis including the partial separation of some species inside individual classes. Mass spectra with high mass accuracy and resolving power are acquired using ESI in both positive- and negative-ion modes for the identification of individual species. The composition of esterified fatty acids and polar head groups are characterized based on their characteristic fragment ions obtained from data independent analysis with high and low collision energy. IM is used as an additional separation dimension enabling the separation of lipid classes together with the separation of individual lipid species differing in acyl chain lengths and the number of double bonds coeluting in SFC. Developed SFC/IM-MS method is applied for the comprehensive analysis of lipid composition
Czech name
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Czech description
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Project
<a href="/en/project/LL1302" target="_blank" >LL1302: Mass Spectrometry in Cancer Research: Lipid Biomarkers for Early Diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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