Comparison of SFC/MS and UHPLC/MS techniques in the lipidomic characterization of biological samples

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899343" target="_blank" >RIV/00216275:25310/14:39899343 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Comparison of SFC/MS and UHPLC/MS techniques in the lipidomic characterization of biological samples
Original language description
Nowadays, supercritical fluid chromatography (SFC) has a renaissance in analytical chemistry due to the commercial availability of reliable SFC systems with potential applications for fast separations of compounds with low to medium polarity, such as lipids. SFC / mass spectrometry (SFC/MS) coupling brings some advantages over conventional (U)HPLC/MS systems, especially in terms of reduced analysis time and therefore higher throughput in the lipidomic analysis. The basic approach for the lipidomic quantitation is based on the direct infusion (shotgun) without any chromatography, but this technique could suffer from suppression effects and the lack of structural details, such as the differentiation of isobaric species. (U)HPLC/MS can be used either forlipid class or lipid species separation. Lipid class separation is achieved by hydrophilic-liquid chromatography (HILIC) for polar lipids or normal-phase (NP) HPLC for nonpolar lipids. Reversed-phase (RP) HPLC enables the resolution of li
Czech name
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Czech description
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Project
<a href="/en/project/LL1302" target="_blank" >LL1302: Mass Spectrometry in Cancer Research: Lipid Biomarkers for Early Diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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