New lipidomics approach: High-throughput nontargeted lipidomic analysis using supercritical fluid chromatography/ion-mobility mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899335" target="_blank" >RIV/00216275:25310/14:39899335 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
New lipidomics approach: High-throughput nontargeted lipidomic analysis using supercritical fluid chromatography/ion-mobility mass spectrometry
Original language description
Lipidomic analysis is challenging due to enormous complexity of lipid structures. Two well-established analytical approaches are commonly used in lipidomics, such as shotgun ESI-MS/MS and LC/MS, both having some advantages and limitations. Alternative high-throughput and nontargeted approach is developed in this work using supercritical fluid chromatography (SFC) followed by ESI and ion mobility-mass spectrometry (IM-MS) for the determination of lipid composition. Individual parameters of SFC analysis have been carefully optimized to achieve a maximum number of separated lipid classes. Developed SFC method enables the separation of 26 nonpolar and polar lipid classes within 6 minutes. Mass spectra with high mass accuracy and resolving power are acquired using ESI in both positive- and negative-ion modes for the identification and quantitation of individual species. The quantitative analysis is performed using internal standards for each lipid class. IM is used as an additional separati
Czech name
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Czech description
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Project
<a href="/en/project/LL1302" target="_blank" >LL1302: Mass Spectrometry in Cancer Research: Lipid Biomarkers for Early Diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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