Development of Comprehensive LC/MS Approach for Clinical Lipidomic Applications and Biomarker Discovery

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899344" target="_blank" >RIV/00216275:25310/14:39899344 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Development of Comprehensive LC/MS Approach for Clinical Lipidomic Applications and Biomarker Discovery
Original language description
Lipidomics is used in clinical studies to improve the understanding how lipids function in biological systems and for the elucidation of the mechanism of lipid-related diseases. We are developing a comprehensive lipidomic approach based on the combination of several liquid chromatography - mass spectrometry (LC/MS) based methods. Total lipid extracts are separated into individual lipid classes using hydrophilic interaction liquid chromatography (HILIC) [1] or supercritical fluid chromatography (SFC) andquantified by response factor approach for lipid classes [2]. HILIC-LC/MS method enables the separation of multiple polar lipid classes (such as different classes of phospholipids, sphingolipids and glycolipids), but unfortunately nonpolar lipid classescoelute in the void volume of the system, so another normal-phase LC/MS method has been developed for the quantitation of tri-, di- and monoacylglycerols, cholesterol esters and cholesterol. SFC/MS is enables the separation of both polar
Czech name
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Czech description
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Project
<a href="/en/project/LL1302" target="_blank" >LL1302: Mass Spectrometry in Cancer Research: Lipid Biomarkers for Early Diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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