Nontargeted quantitation of lipid classes using hydrophilic interaction liquid chromatography - electrospray ionization mass spectrometry with single internal standard and response factor approach
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F12%3A50000940" target="_blank" >RIV/62690094:18470/12:50000940 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/ac3024476" target="_blank" >http://dx.doi.org/10.1021/ac3024476</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/ac3024476" target="_blank" >10.1021/ac3024476</a>
Alternative languages
Result language
angličtina
Original language name
Nontargeted quantitation of lipid classes using hydrophilic interaction liquid chromatography - electrospray ionization mass spectrometry with single internal standard and response factor approach
Original language description
The identification and quantitation of a wide range of lipids in complex biological samples is an essential requirement for the lipidomic studies. High-performance liquid chromatography-mass spectrometry (HPLC/MS) has the highest potential to obtain detailed information on the whole lipidome, but the reliable quantitation of multiple lipid classes is still a challenging task. In this work, we describe a new method for the nontargeted quantitation of polar lipid classes separated by hydrophilic interaction liquid chromatography (HILIC) followed by positive-ion electrospraz ionization mass spectrometry (ESI-MS) using a single internal lipid standard to which all class specific response factors (RFs) are related to. The developed method enables the nontargeted quantitation of lipid classes and molecules inside these classes in contrast to the conventional targeted quantitation, which is based on predefined selected reaction monitoring (SRM) transitions for selected lipids only. In the non
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical chemistry
ISSN
0003-2700
e-ISSN
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Volume of the periodical
84
Issue of the periodical within the volume
22
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
10064-10070
UT code for WoS article
000312434800056
EID of the result in the Scopus database
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