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Nontargeted quantitation of lipid classes using hydrophilic interaction liquid chromatography - electrospray ionization mass spectrometry with single internal standard and response factor approach

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F12%3A50000940" target="_blank" >RIV/62690094:18470/12:50000940 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1021/ac3024476" target="_blank" >http://dx.doi.org/10.1021/ac3024476</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1021/ac3024476" target="_blank" >10.1021/ac3024476</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Nontargeted quantitation of lipid classes using hydrophilic interaction liquid chromatography - electrospray ionization mass spectrometry with single internal standard and response factor approach

  • Original language description

    The identification and quantitation of a wide range of lipids in complex biological samples is an essential requirement for the lipidomic studies. High-performance liquid chromatography-mass spectrometry (HPLC/MS) has the highest potential to obtain detailed information on the whole lipidome, but the reliable quantitation of multiple lipid classes is still a challenging task. In this work, we describe a new method for the nontargeted quantitation of polar lipid classes separated by hydrophilic interaction liquid chromatography (HILIC) followed by positive-ion electrospraz ionization mass spectrometry (ESI-MS) using a single internal lipid standard to which all class specific response factors (RFs) are related to. The developed method enables the nontargeted quantitation of lipid classes and molecules inside these classes in contrast to the conventional targeted quantitation, which is based on predefined selected reaction monitoring (SRM) transitions for selected lipids only. In the non

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Analytical chemistry

  • ISSN

    0003-2700

  • e-ISSN

  • Volume of the periodical

    84

  • Issue of the periodical within the volume

    22

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    7

  • Pages from-to

    10064-10070

  • UT code for WoS article

    000312434800056

  • EID of the result in the Scopus database