Non-Targeted Quantitation of Lipid Classes Using Hydrophilic Interaction Liquid Chromatography - Electrospray Ionization Mass Spectrometry Using Single Internal Standard and Response Factor Approach

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39895379" target="_blank" >RIV/00216275:25310/12:39895379 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/ac3024476" target="_blank" >http://dx.doi.org/10.1021/ac3024476</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/ac3024476" target="_blank" >10.1021/ac3024476</a>

Result language
angličtina
Original language name
Non-Targeted Quantitation of Lipid Classes Using Hydrophilic Interaction Liquid Chromatography - Electrospray Ionization Mass Spectrometry Using Single Internal Standard and Response Factor Approach
Original language description
The identification and quantitation of a wide range of lipids in complex biological samples is an essential requirement for the lipidomic studies. High-performance liquid chromatography?mass spectrometry (HPLC/MS) has the highest potential to obtain detailed information on the whole lipidome, but the reliable quantitation of multiple lipid classes is still a challenging task. In this work, we describe a new method for the nontargeted quantitation of polar lipid classes separated by hydrophilic interaction liquid chromatography (HILIC) followed by positive-ion electrospray ionization mass spectrometry (ESI-MS) using a single internal lipid standard to which all class specific response factors (RFs) are related to. The developed method enables the nontargeted quantitation of lipid classes and molecules inside these classes in contrast to the conventional targeted quantitation, which is based on predefined selected reaction monitoring (SRM) transitions for selected lipids only. In the non
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
<a href="/en/project/GAP206%2F11%2F0022" target="_blank" >GAP206/11/0022: Lipidomic profiling of patients with cardiovascular diseases</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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