Clostridial collagenase immobilized on chitosan nanofibers for burn healing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F22%3A39918517" target="_blank" >RIV/00216275:25310/22:39918517 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/22:10444659

Result on the web

<a href="https://mmsl.cz/corproof.php?tartkey=mms-000000-0205" target="_blank" >https://mmsl.cz/corproof.php?tartkey=mms-000000-0205</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.31482/mmsl.2022.012" target="_blank" >10.31482/mmsl.2022.012</a>

Result language

angličtina

Original language name

Clostridial collagenase immobilized on chitosan nanofibers for burn healing

Original language description

This article describes the characterization and application of collagenase-based chitosan nanofiber membranes with rat burns. Electrospun chitosan nanofibers were functionalized with clostridial collagenase using carbodiimide chemistry. The immobilized collagenase was characterized by enzyme activity, kinetic constants, and dry storage stability measurements using a Pz-peptide substrate. The apparent kinetic constants KM and Vmax of immobilized collagenase showed a high affinity for the peptide substrate compared to the free enzyme. Drying of chitosan membranes with immobilized collagenase ensured 98% stability of enzyme activity after rehydration. The effect of collagenase immobilized on chitosan nanofibers on the burn of the rat model was compared with a control treatment with chitosan nanofibers. The healing of the wound with both materials was terminated after 30 days at the same time, although the collagenase wound healed more rapidly during healing. The scar area size after the application of collagenase-containing chitosan nanofiber membranes was 31.6% smaller than when only chitosan nanofibers were used.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Military Medical Science Letters

ISSN

0372-7025

e-ISSN

2571-113X

Volume of the periodical

91

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

324-334

UT code for WoS article

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EID of the result in the Scopus database

2-s2.0-85143291488