TESTING OF COLLAGENASE COVALENTLY LINKED TO CHITOSAN NANOFIBERS FOR BIOMEDICAL APPLICATIONS

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39899455" target="_blank" >RIV/00216275:25310/15:39899455 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
TESTING OF COLLAGENASE COVALENTLY LINKED TO CHITOSAN NANOFIBERS FOR BIOMEDICAL APPLICATIONS
Original language description
Nanofibers biofunctionalized by enzymes is materials of interest in the biomedical field due to their potential application in wound healing. This work describes the production and characterisation of electrospun chitosan nanofibers biofunctionalized bymicrobial collagenase. The morphology and microstructure of the chitosan nanofibers prepared by Nanospider technology were examined using a scanning electron microscopy (SEM). Amino groups of chitosan nanofiber were connected with the carboxylic acid groups of collagenase using EDAC and sulfo-NHS methodology. Collagenase enzymatic activity was evaluated by peptidic substrate (Pz-peptide, 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg) in order to estimate the ability of this biomaterial to be used as an enzymatic debriding wound dressing. Characterisation of collagenase chitosan nanofibers in term of apparent Michaelis-Menten constants (KM(app) and vmax) measurements revealed higher enzyme to substrate affinity in comparison with so
Czech name
—
Czech description
—

Project
—
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)