Biosensor with electrochemically active nanocomposites for signal amplification and simultaneous detection of three ovarian cancer biomarkers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F23%3A39920862" target="_blank" >RIV/00216275:25310/23:39920862 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/23:PU149658
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0013468623013853?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0013468623013853?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2023.143213" target="_blank" >10.1016/j.electacta.2023.143213</a>
Alternative languages
Result language
angličtina
Original language name
Biosensor with electrochemically active nanocomposites for signal amplification and simultaneous detection of three ovarian cancer biomarkers
Original language description
The current trend in medical diagnostic methods is to simplify and accelerate diagnosis and bring testing closer to the patient while maintaining the existing analytical criteria. Point-of-care tests (POCT) allow improved screening of population and rapid decisions regarding patient care. In case of cancer, this is a very desirable and valuable factor affecting the survival rate. Biosensors exploiting the bioaffinity interaction of target molecules with specific antibodies, combined with screen-printed electrodes as detection units, meet these criteria perfectly. In this study, we present a new sandwich-type magneto-immunosensor with electrochemical signal generating nanocomposites that was designed for the simultaneous detection and quantification of three biomarkers associated with ovarian cancer, namely human epididymis protein 4 (HE4), alpha-fetoprotein (AFP), and cancer antigen 125 (CA-125). The simultaneous detection was enabled by three different electroactive nanomaterials, namely gold nanoparticles (AuNPs), and CdTe and PbS core-type quantum dots (QDs), which were conjugated with three specific antibodies. Mesoporous silica nanoparticles (SiNPs) were incorporated in nanocomposite for higher loading of electroactive labels, leading to substantial enhancement of electrochemical signal. Square-wave anodic/cathodic stripping voltammetry was used for the detection of metal ions released quantitatively from the captured bioconjugates. Combination of these three elements provided separate non interfering signals, hence enabling simultaneous detection. Using this multiplex immunosensor, up to three biomarkers within the linear concentration ranges of 0.02-20 pM for HE4, 0.45-450 IU L 1 for CA-125, and 0.1-500 ng L 1 for AFP could be detected and quantified. The analytical parameters obtained fit the requirements of early-stage disease detection.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrochimica Acta
ISSN
0013-4686
e-ISSN
1873-3859
Volume of the periodical
469
Issue of the periodical within the volume
November
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
143213
UT code for WoS article
001097479700001
EID of the result in the Scopus database
2-s2.0-85172162880