Mitochondrial damage precedes the changes of glutathione metabolism in CdCl2 treated neuronal SH-SY5Y cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F24%3A39921750" target="_blank" >RIV/00216275:25310/24:39921750 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S0278691524005192?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0278691524005192?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fct.2024.114953" target="_blank" >10.1016/j.fct.2024.114953</a>
Alternative languages
Result language
angličtina
Original language name
Mitochondrial damage precedes the changes of glutathione metabolism in CdCl2 treated neuronal SH-SY5Y cells
Original language description
Cadmium crosses the blood-brain barrier inducing damage to neurons. Cell impairment is predominantly linked to oxidative stress and glutathione (GSH) depletion. On the other hand, several reports have described an increase of GSH levels in neuronal cells after CdCl2 exposure. Therefore, the aim of the present report was to investigate the relation between changes in GSH levels and mitochondrial damage in neuronal cells after CdCl2 treatment. To characterize neuronal impairment after CdCl2 treatment (0-200 mu M) for 1-48 h, we used the SH-SY5Y cell line. We analyzed GSH metabolism and determined mitochondrial activity using high-resolution respirometry. CdCl2 treatment induced both the decreases and increases of GSH levels in SH-SY5Y cells. GSH concentration was significantly increased in cells incubated with up to 50 mu M CdCl2 but only 100 mu M CdCl2 induced GSH depletion linked to increased ROS production. The overexpression of proteins involved in GSH synthesis increased in response to 50 and 100 mu M CdCl2 after 6 h. Finally, strong mitochondrial impairment was detected even in 50 mu M CdCl2 treated cells after 24 h. We conclude that a significant decrease in mitochondrial activity can be observed in 50 mu M CdCl2 even without the occurrence of GSH depletion in SH-SY5Y cells.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
21101 - Food and beverages
Result continuities
Project
<a href="/en/project/EF18_069%2F0010054" target="_blank" >EF18_069/0010054: IT4Neuro(degeneration)</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Food and Chemical Toxicology
ISSN
0278-6915
e-ISSN
1873-6351
Volume of the periodical
193
Issue of the periodical within the volume
November 2024
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
114953
UT code for WoS article
001316970200001
EID of the result in the Scopus database
2-s2.0-85202788137