Comprehensive Quantitative Profiling of Less Polar Lipids in Human Plasma Using Validated Reversed-Phase UHPSFC/MS/MS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F25%3A39923030" target="_blank" >RIV/00216275:25310/25:39923030 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1021/acs.analchem.5c04668" target="_blank" >https://doi.org/10.1021/acs.analchem.5c04668</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.5c04668" target="_blank" >10.1021/acs.analchem.5c04668</a>
Alternative languages
Result language
angličtina
Original language name
Comprehensive Quantitative Profiling of Less Polar Lipids in Human Plasma Using Validated Reversed-Phase UHPSFC/MS/MS
Original language description
The quantitative analysis of lipids is challenging due to their structural diversity and the coexistence of numerous isomers in biological samples. Here, we present a robust and validated method based on reversed-phase ultrahigh-performance supercritical fluid chromatography-tandem mass spectrometry (RP-UHPSFC/MS/MS) for the high-throughput profiling of less polar lipids in human plasma. The sample preparation workflow involves an initial Folch-based extraction, followed by chemical derivatization with benzoyl chloride, and the final liquid–liquid extraction into hexane to isolate less polar acylated analytes. This strategy enables the sensitive profiling of six major lipid classes (TG, DG, MG, SE, ST, and FA) in positive ion mode in less than 18 min. The use of two C18 columns in series provides high chromatographic resolution, sufficient to separate isomeric species, including cis/trans and positional isomers of double bonds in fatty acids. The method was rigorously validated for five lipid classes, except free FA, and the implementation of response factors was shown to be essential for the accurate quantification of sterol esters. A total of 147 lipid species were quantified in NIST SRM 1950 human plasma, demonstrating its suitability for large-scale lipidomic studies focused on less polar lipids.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10400 - Chemical sciences
Result continuities
Project
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Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
1520-6882
Volume of the periodical
97
Issue of the periodical within the volume
48
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
26501-26510
UT code for WoS article
001621964800001
EID of the result in the Scopus database
2-s2.0-105024669803