Determination of one year stability of lipid plasma profile and comparison of blood collection tubes using UHPSFC/MS and HILIC-UHPLC/MS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73603912" target="_blank" >RIV/61989592:15110/20:73603912 - isvavai.cz</a>

Alternative codes found

RIV/00216275:25310/20:39916266

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0003267020309089" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0003267020309089</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2020.08.061" target="_blank" >10.1016/j.aca.2020.08.061</a>

Result language

angličtina

Original language name

Determination of one year stability of lipid plasma profile and comparison of blood collection tubes using UHPSFC/MS and HILIC-UHPLC/MS

Original language description

Effects of blood collection tubes, the time period, the sample origin, and the method used on the lipidomic profile are investigated by ultrahigh-performance supercritical fluid chromatography mass spectrometry (UHPSFC/MS) and hydrophilic interaction liquid chromatography ultrahigh-performance liquid chromatography mass spectrometry (HILIC-UHPLC/MS). Heparin plasma samples have been obtained from 99 healthy volunteers at three time points separated by six-month intervals together with one collection for EDTA plasma and serum. Furthermore, lipid concentrations in heparin plasma collected at two different sites are compared. 171 lipid species from eight lipid classes are quantified with UHPSFC/ MS, and 122 lipid species from four lipid classes with HILIC-UHPLC/MS. The accuracy of both methods is monitored by the quantitation error using two internal standards (IS) per individual lipid classes. No significant differences in lipid profiles are observed for different time points and types of collection tubes (heparin plasma, EDTA plasma, and serum). Most pronounced lipid concentration differences are observed for the comparison of NIST SRM 1950 human plasma and mean lipid concentrations of the investigated cohort. Furthermore, differences in lipid concentrations are observed between employed methods (UHPSFC/MS vs. HILIC-UHPLC/MS), which can be compensated by the normalization using NIST SRM 1950 human plasma used as the quality control sample. (c) 2020 Elsevier B.V. All rights reserved.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30204 - Oncology

Project

<a href="/en/project/GJ20-23290Y" target="_blank" >GJ20-23290Y: Supercritical fluid chromatography hyphenated to mass spectrometry for the absolute quantitation of ionic and polar biomolecules</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ANALYTICA CHIMICA ACTA

ISSN

0003-2670

e-ISSN

—

Volume of the periodical

1137

Issue of the periodical within the volume

November 2020

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

74-84

UT code for WoS article

000595332500009

EID of the result in the Scopus database

2-s2.0-85090714631