TiO2 P25 nanoparticles induce mitochondrial damage and increased glutathione synthesis in SH-SY5Y neural cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F25%3A39923138" target="_blank" >RIV/00216275:25310/25:39923138 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.fct.2025.115496" target="_blank" >https://doi.org/10.1016/j.fct.2025.115496</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fct.2025.115496" target="_blank" >10.1016/j.fct.2025.115496</a>
Alternative languages
Result language
angličtina
Original language name
TiO2 P25 nanoparticles induce mitochondrial damage and increased glutathione synthesis in SH-SY5Y neural cells
Original language description
The increasing occurrence of TiO2 nanoparticles in the environment leads to a higher risk of their entry into the human body. Because the nanomaterials can cross the blood-brain barrier, the knowledge of their effect in neural cells is also crucial. However, the exact mechanism of toxicity remains unclear. Therefore, our objective was to evaluate the biological effect of TiO2 nanoparticles in neural cells. Therefore, we aimed to evaluate the biological effect of TiO2 nanoparticles in neural cells. We used neuroblastoma SH-SY5Y cells treated with 25 nm TiO2 nanoparticles (TiO2 P25; 1-100 µg.mL(-1); 24-72 h). We measured dehydrogenase activity, glutathione metabolism, DNA damage and mitochondrial respiration. After treatment of SH-SY5Y cells with TiO2 P25, we found a dose- and time-dependent decrease in dehydrogenase activity together with nuclear changes. Interestingly, 100 µg.mL(-1) TiO2 P25 caused a significant increase of glutathione levels (p < 0.001) linked with increased glutamate-cysteine ligase expression after both 24 and 48 h. Furthermore, as the first study at all, we observed the significant decreases of mitochondrial respiration in SH-SY5Y cells caused by treatments with 10 and 100 µg.mL(-1) TiO2 P25 after 48 and 72 h. In conclusion, our study brings new finding of occurrence of decreased mitochondrial respiration without glutathione depletion in SH-SY5Y cells contributing to the understanding of TiO2 P25 toxicity in neural cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40500 - Other agricultural sciences
Result continuities
Project
<a href="/en/project/EF17_048%2F0007421" target="_blank" >EF17_048/0007421: Strengthening interdisciplinary cooperation in research of nanomaterials and their effects on living organisms (NANOBIO)</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Food and Chemical Toxicology
ISSN
0278-6915
e-ISSN
1873-6351
Volume of the periodical
202
Issue of the periodical within the volume
August 2025
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
115496
UT code for WoS article
001488077800001
EID of the result in the Scopus database
2-s2.0-105003810333