Mitochondria in Biodosimetry: Flow Cytometry Assessment in Vitro
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25520%2F22%3A39918891" target="_blank" >RIV/00216275:25520/22:39918891 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/rpd/article-abstract/198/9-11/521/6673013" target="_blank" >https://academic.oup.com/rpd/article-abstract/198/9-11/521/6673013</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/rpd/ncac092" target="_blank" >10.1093/rpd/ncac092</a>
Alternative languages
Result language
angličtina
Original language name
Mitochondria in Biodosimetry: Flow Cytometry Assessment in Vitro
Original language description
The JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. The probe was tested in vitro on two established cell lines and peripheral porcine blood lymphocytes after gamma irradiation (IR) to assess its potential in biodosimetric evaluation. In brief, we stained irradiated and non-irradiated cells with the JC-1 dye to determine the existing changes in mitochondrial membrane potential and monitor cell health through flow cytometry. The stage of injury in these cells was evaluated through an irradiated versus non-irradiated ratio (IVNIR), comparing the relative proportion of polarised cells containing red JC-1 aggregates. We observed a decreasing IVNIR as the radiation dose increased (i.e. 0.5; 1; 2; 4; 6; 8 and 10 Gy), performing the analysis at 4, 8 and 24 h after IR in all the tested cells. The results from the JC1-dye test showed that CD4 T lymphocytes were more sensitive to irradiation than other subpopulations.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30224 - Radiology, nuclear medicine and medical imaging
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Radiation Protection Dosimetry
ISSN
0144-8420
e-ISSN
1742-3406
Volume of the periodical
198
Issue of the periodical within the volume
9-11
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
521-526
UT code for WoS article
000844405700005
EID of the result in the Scopus database
2-s2.0-85137124613