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Mitochondria in Biodosimetry: Flow Cytometry Assessment in Vitro

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25520%2F22%3A39918891" target="_blank" >RIV/00216275:25520/22:39918891 - isvavai.cz</a>

  • Result on the web

    <a href="https://academic.oup.com/rpd/article-abstract/198/9-11/521/6673013" target="_blank" >https://academic.oup.com/rpd/article-abstract/198/9-11/521/6673013</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1093/rpd/ncac092" target="_blank" >10.1093/rpd/ncac092</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Mitochondria in Biodosimetry: Flow Cytometry Assessment in Vitro

  • Original language description

    The JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. The probe was tested in vitro on two established cell lines and peripheral porcine blood lymphocytes after gamma irradiation (IR) to assess its potential in biodosimetric evaluation. In brief, we stained irradiated and non-irradiated cells with the JC-1 dye to determine the existing changes in mitochondrial membrane potential and monitor cell health through flow cytometry. The stage of injury in these cells was evaluated through an irradiated versus non-irradiated ratio (IVNIR), comparing the relative proportion of polarised cells containing red JC-1 aggregates. We observed a decreasing IVNIR as the radiation dose increased (i.e. 0.5; 1; 2; 4; 6; 8 and 10 Gy), performing the analysis at 4, 8 and 24 h after IR in all the tested cells. The results from the JC1-dye test showed that CD4 T lymphocytes were more sensitive to irradiation than other subpopulations.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30224 - Radiology, nuclear medicine and medical imaging

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Radiation Protection Dosimetry

  • ISSN

    0144-8420

  • e-ISSN

    1742-3406

  • Volume of the periodical

    198

  • Issue of the periodical within the volume

    9-11

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    6

  • Pages from-to

    521-526

  • UT code for WoS article

    000844405700005

  • EID of the result in the Scopus database

    2-s2.0-85137124613