Digital holographic microscope with low-spatial and temporal coherence of illumination
Result description
In this paper we present a newly developed digital transmission holographic microscope. The microscope enables using arbitrarily low coherent illumination (both spatially and temporally) in conjunction with the off-axis holography. The setup of the microscope, its function and the object wave reconstruction procedure are described. The optical sectioning effect, similar to a confocal microscope, resulting from the use of low spatially coherent light source is demonstrated. The microscope has been tailored for studies of living cell dynamics. Time-lapse phase reconstruction series of live cells activities were carried out. The different behavior related to changes in the cell cycle is demonstrated.
Keywords
digital imagingholographylow coherenceimage reconstructionlive cells
The result's identifiers
Result code in IS VaVaI
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Digital holographic microscope with low-spatial and temporal coherence of illumination
Original language description
In this paper we present a newly developed digital transmission holographic microscope. The microscope enables using arbitrarily low coherent illumination (both spatially and temporally) in conjunction with the off-axis holography. The setup of the microscope, its function and the object wave reconstruction procedure are described. The optical sectioning effect, similar to a confocal microscope, resulting from the use of low spatially coherent light source is demonstrated. The microscope has been tailored for studies of living cell dynamics. Time-lapse phase reconstruction series of live cells activities were carried out. The different behavior related to changes in the cell cycle is demonstrated.
Czech name
Digitální holografická mikroskopie s nízkou prostorovou a časovou koherencí osvětlení
Czech description
V tomto článku prezentujeme nově vyvinutý transmisní digitální holografický mikroskop. Mikroskop umožňuje použití světla s různou koherencí (jak časovou, tak I prostorovou) současně s mimoosovou holografií. Je popsáno schema mikroskopu, jeho funkce a rekonstrukce objektové vlny. Je demonstrován jev optických řezů (podobný tomu v konfokálním mikroskopu), který je důsledkem použití světla s nízkou kohernecí. Mikroskop byl použit pro stadium dynamiky živých buněk. Různá chování související se změnami buněčného cyklu jsou zde demonstrovány.
Classification
Type
D - Article in proceedings
CEP classification
BH - Optics, masers and lasers
OECD FORD branch
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Result continuities
Project
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Optical and Digital Image Processing
ISBN
978-0-8194-7198-7
ISSN
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e-ISSN
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Number of pages
8
Pages from-to
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Publisher name
SPIE
Place of publication
Washington 98227-0010 USA
Event location
Strasbourg
Event date
Apr 7, 2008
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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Basic information
Result type
D - Article in proceedings
CEP
BH - Optics, masers and lasers
Year of implementation
2008