System for coherence-controlled holographic microscopy of living cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F12%3APU102376" target="_blank" >RIV/00216305:26210/12:PU102376 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
System for coherence-controlled holographic microscopy of living cells
Original language description
Coherence Controlled Holographic Microscopy (CCHM) is a novel holographic technique for quantitative-phase contrast (QPC) biological observations particularly of living cells. Owing to the ordinary (low coherence) illumination source, the CCHM images areof low noise, deprived of coherence noise (speckles) and the lateral resolution is improved by a factor of 2 compared to classic holographic microscopes. Long-lasting time-lapse experiments require elimination of the CCHM optical system instability in order to achieve precise QPC measurement and to maintain correct CCHM adjustment for its low-coherence operation. The critical part of CCHM is the interferometer, which is very sensitive to temperature fluctuations and air turbulences. The temperature stabilization of the whole microscope without air turbulences is therefore required to provide stability for long-term observations of living cells. Novel heated microscope box and stage designed and constructed for this purpose are describe
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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