Coherence-controlled holographic microscopy for live-cell quantitative phase imaging

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F15%3APU114546" target="_blank" >RIV/00216305:26210/15:PU114546 - isvavai.cz</a>
Result on the web
<a href="http://spie.org/Publications/Proceedings/Paper/10.1117/12.2080128" target="_blank" >http://spie.org/Publications/Proceedings/Paper/10.1117/12.2080128</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/12.2080128" target="_blank" >10.1117/12.2080128</a>

Result language
angličtina
Original language name
Coherence-controlled holographic microscopy for live-cell quantitative phase imaging
Original language description
In this paper we present coherence-controlled holographic microscopy (CCHM) and various examples of observations of living cells including combination of CCHM with fluorescence microscopy. CCHM is a novel technique of quantitative phase imaging (QPI). Itis based on grating off-axis interferometer, which is fully adapted for the use of incoherent illumination. This enables high-quality QPI free from speckles and parasitic interferences and lateral resolution of classical widefield microscopes. Label-free nature of QPI makes CCHM a useful tool for long-term observations of living cells. Moreover, coherence-gating effect induced by the use of incoherent illumination enables QPI of cells even in scattering media. Combination of CCHM with common imaging techniques brings the possibility to exploit advantages of QPI while simultaneously identifying the observed structures or processes by well-established imaging methods. We used CCHM for investigation of general parameters of cell life cycl
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Quantitative Phase Imaging
ISBN
978-1-62841-426-4
ISSN
—
e-ISSN
—
Number of pages
4
Pages from-to
"93360K-1"-"93360K-4"
Publisher name
SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
Place of publication
San Francisco, CA
Event location
San Francisco
Event date
Feb 7, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000354170000008

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