Quantitative phase imaging through scattering media

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F15%3APU114498" target="_blank" >RIV/00216305:26620/15:PU114498 - isvavai.cz</a>

Result on the web

<a href="http://spie.org/Publications/Proceedings/Paper/10.1117/12.2078003" target="_blank" >http://spie.org/Publications/Proceedings/Paper/10.1117/12.2078003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.2078003" target="_blank" >10.1117/12.2078003</a>

Result language

angličtina

Original language name

Quantitative phase imaging through scattering media

Original language description

Coherence-controlled holographic microscope (CCHM) is an off-axis holographic system. It enables observation of a sample and its quantitative phase imaging with coherent as well as with incoherent illumination. The spatial and temporal coherence can be modified and thus also the quality and type of the image information. The coherent illumination provides numerical refocusing in wide depth range similarly to a classic coherent-light digital holographic microscopy (HM). Incoherent-light HM is characterized by a high quality, coherence-noise-free imaging with up to twice higher resolution compared to coherent illumination. Owing to an independent, free of sample reference arm of the CCHM the low spatial light coherence induces coherence-gating effect. This makes possible to observe specimen also through scattering media. We have described theoretically and simulated numerically imaging of a two dimensional object through a scattering layer by CCHM using the linear systems theory. We have investigated both strongly and weakly scattering media characterized by different amount of ballistic and diffuse light. The influence of a scattering layer on the quality of a phase signal is discussed for both types of the scattering media. A strong dependence of the imaging process on the light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with model samples, as well as real biologic objects particularly then by time-lapse observations of live cells reactions to substances producing optically turbid emulsion.

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

BH - Optics, masers and lasers

OECD FORD branch

—

Project

<a href="/en/project/EE2.3.30.0039" target="_blank" >EE2.3.30.0039: Excellent young researcher at BUT</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Quantitative Phase Imaging

ISBN

978-1-62841-426-4

ISSN

—

e-ISSN

—

Number of pages

8

Pages from-to

"93360T-1"-"93360T-8"

Publisher name

SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA

Place of publication

San Francisco, CA

Event location

San Francisco

Event date

Feb 7, 2015

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000354170000013