Modelling invasive pathogen load from non-destructive sampling data
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F19%3APU131273" target="_blank" >RIV/00216305:26210/19:PU131273 - isvavai.cz</a>
Alternative codes found
RIV/68081766:_____/19:00499378 RIV/62157124:16270/19:43877448 RIV/00216224:14110/19:00113549
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0022519318306258?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0022519318306258?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jtbi.2018.12.026" target="_blank" >10.1016/j.jtbi.2018.12.026</a>
Alternative languages
Result language
angličtina
Original language name
Modelling invasive pathogen load from non-destructive sampling data
Original language description
Where microbes colonizing skin surface may help maintain organism homeostasis, those that invade living skin layers cause disease. In bats, white-nose syndrome is a fungal skin infection that affects animals during hibernation and may lead to mortality in severe cases. Here, we inferred the amount of fungus that had invaded skin tissue of diseased animals. We used simulations to estimate the unobserved disease severity in a non-lethal wing punch biopsy and to relate the simulated pathology to the measured fungal load in paired biopsies. We found that a single white-nose syndrome skin lesion packed with spores and hyphae of the causative agent, Pseudogymnoascus destructans, contains 48.93 pg of the pathogen DNA, which amounts to about 1560 P destructans genomes in one skin lesion. Relating the information to the known UV fluorescence in Nearctic and Palearctic bats shows that Nearctic bats carry about 1.7 mu g of fungal DNA per cm(2), whereas Palearctic bats have 0.04 g cm(-2) of P. destructans DNA. With the information on the fungal load that had invaded the host skin, the researchers can now calculate disease severity as a function of invasive fungal growth using non-destructive UV light transillumination of each bat's wing membranes. Our results will enable and promote thorough disease severity assessment in protected bat species without the need for extensive animal and laboratory labor sacrifices.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10102 - Applied mathematics
Result continuities
Project
<a href="/en/project/GA17-20286S" target="_blank" >GA17-20286S: Physiology of bat hibernation with respect to multistressor impacts</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Theoretical Biology
ISSN
0022-5193
e-ISSN
1095-8541
Volume of the periodical
neuveden
Issue of the periodical within the volume
464
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
98-103
UT code for WoS article
000457204500009
EID of the result in the Scopus database
2-s2.0-85059111717