Modelling invasive pathogen load from non-destructive sampling data

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F19%3A00499378" target="_blank" >RIV/68081766:_____/19:00499378 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26210/19:PU131273 RIV/62157124:16270/19:43877448 RIV/00216224:14110/19:00113549

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0022519318306258?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0022519318306258?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jtbi.2018.12.026" target="_blank" >10.1016/j.jtbi.2018.12.026</a>

Result language

angličtina

Original language name

Modelling invasive pathogen load from non-destructive sampling data

Original language description

Where microbes colonizing skin surface may help maintain organism homeostasis, those that invade liv- ing skin layers cause disease. In bats, white-nose syndrome is a fungal skin infection that affects animals during hibernation and may lead to mortality in severe cases. Here, we inferred the amount of fungus that had invaded skin tissue of diseased animals. We used simulations to estimate the unobserved disease severity in a non-lethal wing punch biopsy and to relate the simulated pathology to the measured fungal load in paired biopsies. We found that a single white-nose syndrome skin lesion packed with spores and hyphae of the causative agent, Pseudogymnoascus destructans , contains 48.93 pg of the pathogen DNA, which amounts to about 1560 P destructans genomes in one skin lesion. Relating the information to the known UV fluorescence in Nearctic and Palearctic bats shows that Nearctic bats carry about 1.7 μg of fun- gal DNA per cm 2 , whereas Palearctic bats have 0.04 μg cm −2 of P. destructans DNA. With the information on the fungal load that had invaded the host skin, the researchers can now calculate disease severity as a function of invasive fungal growth using non-destructive UV light transillumination of each bat ʼs wing membranes. Our results will enable and promote thorough disease severity assessment in protected bat species without the need for extensive animal and laboratory labor sacrifices.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Theoretical Biology

ISSN

0022-5193

e-ISSN

—

Volume of the periodical

464

Issue of the periodical within the volume

March

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

98-103

UT code for WoS article

000457204500009

EID of the result in the Scopus database

2-s2.0-85059111717