Advanced Microscopical Non-Invasive Examination of the Supposed Migrastatics for Impact on In Vitro Cell Migration

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F22%3APU144990" target="_blank" >RIV/00216305:26210/22:PU144990 - isvavai.cz</a>
Result on the web
<a href="https://ecop.events/poster-abstracts/" target="_blank" >https://ecop.events/poster-abstracts/</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Advanced Microscopical Non-Invasive Examination of the Supposed Migrastatics for Impact on In Vitro Cell Migration
Original language description
Live H1299 lung carcinoma cells in vitro were exposed to selected drugs with a presumed antimigration activity that implies antimetastatic potential and time-lapse examined with Coherence Controlled Holography Microscopy (CCHM) with holographic incoherent light Quantitive Phase Imaging (hiQPI). It is a methodology that online evaluates the dynamics of morphology, migration, and the growth of tumor cells by weighing them. Material and method: Q-Phase (Telight, Brno, Czech Republic) as a commercially available CCHM was employed for the hiQPI of cells. Four putative migrastatics, vincristine (VIN, 100 nM), doxycycline (DOXY, 1 mg/ml), and 4-hydroxyacetophenone (4HAP, 4 μM) were tested with H1299, using Ibidi μ-Slide VI 0.4 for 20 hours recording with Q-Phase. Cells were cultivated at 37°C in a humidified incubator with 3.5% CO2 in standard Eagle MEM medium with 10% fetal bovine serum, 20 μM gentamicin, and 2mM L-glutamine. For the time-lapse recording, the medium was enriched with 20 mM HEPES to maintain
Czech name
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Czech description
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Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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