Synthetic auxin herbicide 2,4-D and its influence on a model BY-2 suspension

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F24%3APU151174" target="_blank" >RIV/00216305:26210/24:PU151174 - isvavai.cz</a>

Alternative codes found

RIV/86652079:_____/24:00587799

Result on the web

<a href="https://link.springer.com/article/10.1007/s11033-024-09392-x" target="_blank" >https://link.springer.com/article/10.1007/s11033-024-09392-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11033-024-09392-x" target="_blank" >10.1007/s11033-024-09392-x</a>

Result language

angličtina

Original language name

Synthetic auxin herbicide 2,4-D and its influence on a model BY-2 suspension

Original language description

2,4-D is a broadly used auxin herbicide. The presence of the 2,4-D synthetic auxin in the medium is imperative for long-term BY-2 tobacco suspension viability. The precise mechanism of this symbiosis of the suspension and the synthetic auxin remains unclear. Our goal was to study the hormonal regulation of the growth of the cell suspension; and to describe the experiments clarifying the interaction between the chosen growth regulators and phytohormones on the cellular level, specifically between the 2,4-D synthetic auxin and the native stress phytohormone — ethylene. This study examined the influence of low 2,4-D concentrations stimulating cell growth in vitro as well as the influence of high herbicide concentrations on the model tobacco BY-2 suspension. The culture took 6 days. Different parameters were evaluated, including the influence of different 2,4-D concentrations on the production of the phytohormone ethylene and its precursor 1-Aminocyclopropane-1-carboxylic acid (ACC) in the tobacco cells. The content of 2,4-D in the cells and the medium was established. The observations of the morphological changes showed that a heavy impregnation of the cell walls taking place depending on the concentration of 2,4-D. A dramatic increase in protective polysaccharides and a remodulation of the cell walls by the formation of a pectin shield in artificial conditions were expected and observed. At the same time, massive production of the stress phytohormone ethylene took place, and, because of that, plant mutagenicity, anomalous tumour-type proliferation growth, and the production of supercells were observed. The hypothesis of the protective shield is discussed.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

MOLECULAR BIOLOGY REPORTS

ISSN

0301-4851

e-ISSN

1573-4978

Volume of the periodical

51

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

14

Pages from-to

„“-„“

UT code for WoS article

001189632900009

EID of the result in the Scopus database

2-s2.0-85188435813