EMCCD optical mapping system for measurement of action potentials

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F10%3APR24318" target="_blank" >RIV/00216305:26220/10:PR24318 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
EMCCD optical mapping system for measurement of action potentials
Original language description
Optical mapping using membrane potential-sensitive fluorescent dyes provides a means of recording electrical activation on the heart surface with high spatial resolution. However, in order to interpret these data, it is necessary to correct for artifactsintroduced by heart wall motion. The new optical system based on EMCCD camera is designed to be able to record optical signal in high resolution 128x128 at 400fps (via a 550nm> long-pass filter). The system allows use of new ratiometric technique. Imagesequences can be recorded sequentially with and without a second long-pass filter (620nm>) which is introduced into the light path using a fast purpose-built translation system. The system consist of the novel chamber design which allows simultaneouslyrecordings of optical signal on the one side of the heart as well as electrical on another one, all in physiological temperatures. The heart is illuminated with 2 blue LEDs (Luxen V Star, LHXL-LB5C), each equipped with a small reflector,
Czech name
—
Czech description
—

Project
<a href="/en/project/GA102%2F07%2F1473" target="_blank" >GA102/07/1473: Optical Methods for Registration of Heart Electrical Potentials and Calcium Concentration with Laser Stabilization</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Internal product ID
OMS
Numerical identification
—
Technical parameters
Funkční vzorek je využíván na Department of Physiology, Room# 2224, 85 Park Rd..,Grafton, Auckland, New Zealand Fotografie a technicke paramety na webu: http://biomedheartproject.webs.com/ Technicke parametry: Kamera: EMCCD Cascade 128+, 128x128,
Economical parameters
Cena 600000 Kč
Application category by cost
—
Owner IČO
00216305
Owner name
Ústav biomedicínského inženýrství
Owner country
CZ - CZECH REPUBLIC
Usage type
N - Využití výsledku jiným subjektem je možné bez nabytí licence (výsledek není licencován)
Licence fee requirement
—
Web page
—

Similar results(10)