Simultaneous electrical and fluorescence recording of HL-1 cells’ electrical activity in response to extracellular calcium stimulation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F18%3APU129244" target="_blank" >RIV/00216305:26220/18:PU129244 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.22489/CinC.2018.103" target="_blank" >http://dx.doi.org/10.22489/CinC.2018.103</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.22489/CinC.2018.103" target="_blank" >10.22489/CinC.2018.103</a>

Result language

angličtina

Original language name

Simultaneous electrical and fluorescence recording of HL-1 cells’ electrical activity in response to extracellular calcium stimulation

Original language description

In current cell tissue engineering and cardiology, beating models with behavior and properties similar to humans are necessary. The cardiac muscle HL-1 cells, whose genes have a parent with adult atrial myocytes, serve as a suitable model. HL-1 cells were seeded on a 120-electrode microelectrode (MEA) chamber and transient transfection of Accelerated Sensor of Action Potentials 1 (ASAP1), a genetically encoded voltage indicator, was performed. Simultaneous electrical and optical recording of cardiac cell culture electrical activity was made when the cells started to produce spontaneous action potentials. Recording synchronization was made using TTL pulses. Results showed that HL-1 cells start to produce asynchronous spontaneous action potentials (-375±10 µV) when reaching 90% MEA chamber confluency and which become periodical addition of extracellular calcium (-501±14 µV, 2.1±1.0 Hz). The ASAP1’s fluorescent response reached up to 21±5% ΔF/F. The time constant between detection of electrical and fluorescent response (τe/o) was determined as 12±5 ms.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

—

OECD FORD branch

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Computing in Cardiology 2018

ISBN

—

ISSN

2325-887X

e-ISSN

—

Number of pages

4

Pages from-to

1-4

Publisher name

Computing in Cardiology

Place of publication

Maastricht, Netherlands

Event location

Maastricht

Event date

Sep 23, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000482598700162