AC pulsed field ablation is effective and safe in atrial and ventricular settings: a proof-of-concept chronic animal study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F20%3APU137721" target="_blank" >RIV/00216305:26220/20:PU137721 - isvavai.cz</a>

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fbioe.2020.552357/full?&utm_source=Email_to_authors_&utm_medium=Email&utm_content=T1_11.5e1_author&utm_campaign=Email_publication&field=&journalName=Frontiers_in_Bioengineering_and_Biotechnology&id=552357" target="_blank" >https://www.frontiersin.org/articles/10.3389/fbioe.2020.552357/full?&utm_source=Email_to_authors_&utm_medium=Email&utm_content=T1_11.5e1_author&utm_campaign=Email_publication&field=&journalName=Frontiers_in_Bioengineering_and_Biotechnology&id=552357</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fbioe.2020.552357" target="_blank" >10.3389/fbioe.2020.552357</a>

Result language

angličtina

Original language name

AC pulsed field ablation is effective and safe in atrial and ventricular settings: a proof-of-concept chronic animal study

Original language description

Introduction: Pulsed field ablation (PFA) exploits the delivery of short high-power shocks to induce cells death via irreversible electroporation. The therapy offers a potential paradigm shift for catheter ablation of cardiac arrhythmia. We selected the AC-burst as a pulse with optimal combined benefits. We performed a proof-of-concept chronic animal trial to test the feasibility, safety and efficacy of our method and technology. Methods: We employed 6 female swine - weight 53.75±4.77 kg – in this study. With fluoroscopic and electroanatomical mapping assistance, we performed ECG-gated AC-PFA in the following settings: in the left atrium with a decapolar loop catheter with electrodes connected in bipolar fashion; across the interventricular septum applying energy between the distal electrodes of two tip catheters. After procedure and 4-week follow-up, the animals were euthanized, and the hearts were inspected for tissue changes and characterized. We perform finite element method simulation of our AC-PFA scenarios to corroborate our method and better interpret our findings. Results: We applied 60 square AC-bursts of 100 µs duration, 100 kHz internal frequency, 900 V for 60 pulses in the atrium and 1500 V for 120 pulses in the septum. Acute changes in the atrial and ventricular electrograms were immediately visible at the sites of AC-PFA – signals were elongated and reduced in amplitude (p<0.0001) and tissue impedance dropped (p=0.011). No adverse event (e.g. esophageal temperature rises or gas bubble streams) was observed. The implemented numerical simulations confirmed the non-thermal nature of our AC-PFA and provided specific information on the estimated treated area and need of pulse trains. The postmortem chest inspection showed no peripheral damage, but epicardial and endocardial discolorations at sites of ablation. T1-weighted scans revealed specific tissue changes in atria and ventricles, confirmed to be fibrotic scars via trichrome staining. We found isolat

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20601 - Medical engineering

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Bioengineering and Biotechnology

ISSN

2296-4185

e-ISSN

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Volume of the periodical

neuveden

Issue of the periodical within the volume

8

Country of publishing house

CH - SWITZERLAND

Number of pages

27

Pages from-to

1-27

UT code for WoS article

000599322800001

EID of the result in the Scopus database

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