Genomic analysis of Paenibacillus larvae isolates from the Czech Republic and the neighbouring regions of Slovakia
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F23%3APU147888" target="_blank" >RIV/00216305:26220/23:PU147888 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16270/23:43880885 RIV/00027162:_____/23:N0000024
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0034528823000723?utm_campaign=STMJ_AUTH_SERV_PUBLISHED&utm_medium=email&utm_acid=267549703&SIS_ID=&dgcid=STMJ_AUTH_SERV_PUBLISHED&CMX_ID=&utm_in=DM348155&utm_source=AC_" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0034528823000723?utm_campaign=STMJ_AUTH_SERV_PUBLISHED&utm_medium=email&utm_acid=267549703&SIS_ID=&dgcid=STMJ_AUTH_SERV_PUBLISHED&CMX_ID=&utm_in=DM348155&utm_source=AC_</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.rvsc.2023.03.007" target="_blank" >10.1016/j.rvsc.2023.03.007</a>
Alternative languages
Result language
angličtina
Original language name
Genomic analysis of Paenibacillus larvae isolates from the Czech Republic and the neighbouring regions of Slovakia
Original language description
Paenibacillus larvae is the causative agent of American foulbrood (AFB), a devastating disease of honeybee larvae. In the Czech Republic, two large infested regions were recognised. This study aimed to analyse P. larvae strains occurring in the Czech Republic in the years 2016–2017 and to characterise the genetic structure of their population with the use of Enterobacterial Repetitive Intergenic Consensus genotyping (ERIC), multilocus sequence typing (MLST) and whole genome sequence (WGS) analysis. The results were complemented by the analysis of isolates collected in the year 2018 in areas of Slovakia located near the Czechia-Slovakia border. ERIC genotyping revealed that 78.9% of tested isolates belonged to the ERIC II genotype and 21.1% to ERIC I genotype. MLST showed six sequence types with ST10 and ST11 being the most frequent among isolates. Within six isolates we found discrepancies in correlations between MLST and ERIC genotypes. The use of MLST and WGS analysis of isolates revealed that each of the large infested geographic regions had its own dominating P. larvae strains. We assume that these strains represented primary sources of infection in the affected areas. In addition, the sporadic presence of strains identified by core genome analysis as genetically related was unveiled in geographically distant regions suggesting possible human-mediated transmission of AFB.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.
Data specific for result type
Name of the periodical
RESEARCH IN VETERINARY SCIENCE
ISSN
0034-5288
e-ISSN
1532-2661
Volume of the periodical
158
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
34-40
UT code for WoS article
001060254300001
EID of the result in the Scopus database
2-s2.0-85150357322