Inertial co-focusing of heterogeneous particles in hybrid microfluidic channels with constantly variable cross-sections
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F24%3APU152317" target="_blank" >RIV/00216305:26220/24:PU152317 - isvavai.cz</a>
Result on the web
<a href="https://pubs.rsc.org/en/content/articlelanding/2024/lc/d4lc00479e" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2024/lc/d4lc00479e</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/d4lc00479e" target="_blank" >10.1039/d4lc00479e</a>
Alternative languages
Result language
angličtina
Original language name
Inertial co-focusing of heterogeneous particles in hybrid microfluidic channels with constantly variable cross-sections
Original language description
Heterogeneous particles co-focusing to a single stream is a vital prerequisite for cell counting and enumeration, playing an essential role in flow cytometry and single-cell analysis. Microfluidics-based inertial focusing holds great research prospects due to its simplicity of devices, ease of operation, high throughput, and freedom from external fields. Combining microfluidic channels with two or more different geometries has become a powerful tool for high-efficiency particle focusing. Here, we explored hybrid microfluidic channels for heterogeneous particle co-focusing. Four different annular channels with obstacles distributed on the inner wall were constructed and simulated, obtaining constantly variable secondary flows. Then we used four different fluorescent particles with the size of 10 mu m, 12 mu m 15 mu m, and 20 mu m as well as their mixture to perform the inertial focusing experiments of multi-sized particles. Theoretical simulation and experimental results demonstrated a focusing efficiency of >99%. Finally, we further utilized human white blood cells to estimate the co-focusing performance of our hybrid microfluidic channel, resulting in a high focusing efficiency of >92% and a high throughput of approximate to 8000 cell s(-1). The hybrid microfluidic channels, capable of high-precision heterogeneous particle co-focusing, could pave a broad avenue for microfluidic flow cytometry and single-cell analysis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10305 - Fluids and plasma physics (including surface physics)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
LAB ON A CHIP
ISSN
1473-0197
e-ISSN
1473-0189
Volume of the periodical
24
Issue of the periodical within the volume
21
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
1-11
UT code for WoS article
001325091300001
EID of the result in the Scopus database
2-s2.0-85205461945