2DEP cytometry: distributed dielectrophoretic cytometry for live cell dielectric signature measurement on population level

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00669806%3A_____%2F18%3A10367047" target="_blank" >RIV/00669806:_____/18:10367047 - isvavai.cz</a>

Alternative codes found

RIV/49777513:23220/18:43932803 RIV/49777513:23520/18:43932803

Result on the web

<a href="https://link.springer.com/content/pdf/10.1007%2Fs10544-017-0253-5.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs10544-017-0253-5.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10544-017-0253-5" target="_blank" >10.1007/s10544-017-0253-5</a>

Result language

angličtina

Original language name

2DEP cytometry: distributed dielectrophoretic cytometry for live cell dielectric signature measurement on population level

Original language description

In this work, a novel force equilibrium method called distributed dielectrophoretic cytometry (2DEP cytometry) was developed. It uses a dielectrophoresis (DEP)-induced vertical translation of live cells in conjunction with particle image velocimetry (PIV) in order to measure probabilistic distribution of DEP forces acting on an entire cell population. The method is integrated in a microfluidic device. The bottom of the microfluidic channel is lined with an interdigitated electrode array. Cells passing through the micro-channel are acted on by sedimentation forces, while DEP forces either oppose sedimentation, support sedimentation, or neither, depending on the dielectric (DE) signatures of the cells. The heights at which cells stabilize correspond to their DE signature and are measured indirectly using PIV, which enables simultaneous and high-throughput collection of hundreds of single-cell responses in a single PIV frame. The system was validated using polystyrene micro-particles. Preliminary experimental data quantify the DE signatures of immortalized myelogenous leukemia cell lines K562 and KG1. We show DEP-induced cell translation along the parabolic velocity profile can be measured by PIV with sub-micron precision, enabling identification of individual cell DE signatures. DE signatures of the selected cell lines are distinguishable. Throughput of the method enables measurement of DE signatures at 10 different frequencies in almost real time.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Microdevices

ISSN

1387-2176

e-ISSN

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Volume of the periodical

20

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

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UT code for WoS article

000419641400001

EID of the result in the Scopus database

2-s2.0-85043388872