In vitro evaluation of 3D-printed conductive chitosan–polyaniline scaffolds with exosome release for enhanced angiogenesis and cardiomyocyte protection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F26%3A0197944" target="_blank" >RIV/00216305:26220/26:0197944 - isvavai.cz</a>
Result on the web
<a href="https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra02940f" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra02940f</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/D5RA02940F" target="_blank" >10.1039/D5RA02940F</a>
Alternative languages
Result language
angličtina
Original language name
In vitro evaluation of 3D-printed conductive chitosan–polyaniline scaffolds with exosome release for enhanced angiogenesis and cardiomyocyte protection
Original language description
Myocardial infarction (MI) often results in significant damage to heart tissues, leading to cardiac dysfunction, fibrosis, and diminished cell–cell communication. Exosomes (EXOs) from stem cells show great potential in promoting tissue repair and angiogenesis, but their rapid clearance and degradation in vivo limit therapeutic efficacy. Here, we introduce a 3D-printed in vitro scaffold using a conductive biomaterial ink composed of chitosan (CS) and polyaniline (PANI). This scaffold combines the bioactivity of EXOs with the conductive properties of PANI to protect cardiac cells under ischemic stress. Using an in vitro hypoxia/reoxygenation (H/R) model with HL-1 cardiomyocytes, we simulated key aspects of myocardial ischemia-reperfusion injury. The addition of PANI improved the electrical conductivity of the scaffold, which was essential for enhancing cardiomyocyte viability and intercellular connectivity under hypoxic conditions. EXOs significantly promoted angiogenic activity in vitro, as evidenced by enhanced human umbilical vein endothelial cell (HUVEC) migration and robust tube formation, highlighting their role in stimulating new blood vessel growth. Molecular analyses revealed that EXOs positively influence processes such as angiogenesis and inflammation regulation in HL-1 cells. Additionally, EXOs improved HUVEC migration, emphasizing their pro-angiogenic role. These findings indicate that combining PANI and EXOs in a 3D-printed scaffold yields synergistic benefits, improving cardiomyocyte function and promoting endothelial angiogenesis in vitro, thereby providing insights for future cardiac repair strategies.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30404 - Biomaterials (as related to medical implants, devices, sensors)
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
RSC Advances
ISSN
—
e-ISSN
2046-2069
Volume of the periodical
15
Issue of the periodical within the volume
21
Country of publishing house
GB - UNITED KINGDOM
Number of pages
19
Pages from-to
16826-16844
UT code for WoS article
001491002600001
EID of the result in the Scopus database
2-s2.0-105005748873