Collagen-Hyaluronic Acid Hydrogel as a Platform for Modeling Early Stages of Endochondral Ossification In Vitro
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27640%2F25%3A10259644" target="_blank" >RIV/61989100:27640/25:10259644 - isvavai.cz</a>
Result on the web
<a href="https://journals.sagepub.com/doi/10.1177/09636897251409464" target="_blank" >https://journals.sagepub.com/doi/10.1177/09636897251409464</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1177/09636897251409464" target="_blank" >10.1177/09636897251409464</a>
Alternative languages
Result language
angličtina
Original language name
Collagen-Hyaluronic Acid Hydrogel as a Platform for Modeling Early Stages of Endochondral Ossification In Vitro
Original language description
Tissue engineering strategies aim to create bone models to enhance our understanding of bone development and improve bone repair. Endochondral ossification (EO), where cartilage is replaced by bone, is difficult to replicate. We developed a collagen–hyaluronic acid (HA) hydrogel system for a three-dimensional (3D) co-cultivation of human chondrocytes, human mesenchymal stem cell (hMSCs), and human umbilical vein endothelial cells (HUVECs) to model the early stages of EO. To our knowledge, this specific tri-culture configuration within such a hydrogel composition has not been previously reported, providing a novel platform for studying EO-related cellular and matrix interactions in vitro. Differentiation and migration of hMSCs and HUVECs were studied in collagen I (Col I) hydrogels, with or without the addition of collagen II (Col II), HA, and chondrocytes. Col II and HA enhanced cell osteogenic differentiation, as documented by an increased COL1A1 and ALPL gene expression and enhanced alkaline phosphatase activity. Chondrocytes further promoted osteogenic cell differentiation and temporarily enhanced hypertrophic and angiogenic signaling as evidenced by elevated MMP13 and VEGFA expression. Furthermore, elevated SOX9 expression and Alcian blue staining indicated a chondrogenic-supportive environment. These findings highlight the synergistic effects of osteogenic and chondrogenic signals, emphasizing the potential of combining Col I and Col II, HA, and essential cell types in hydrogels to optimize bone and cartilage tissue engineering.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
20500 - Materials engineering
Result continuities
Project
<a href="/en/project/EH22_008%2F0004631" target="_blank" >EH22_008/0004631: Materials and technologies for sustainable development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cell Transplantation
ISSN
0963-6897
e-ISSN
—
Volume of the periodical
35
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
22
Pages from-to
1-22
UT code for WoS article
001689883000001
EID of the result in the Scopus database
2-s2.0-105030225587